LOCATION: Room 513, Clinical Pathology Building (CPB). [MAP]
PHONE NO.: 2855 4178
FAX NO.: 2818 1104
| SERVICE HOURS: | Weekdays 8:40 a.m. - 5:00 p.m. |
| Saturdays 8:40 a.m. - 1:00 p.m. |
Medical Technologist in-charge: Ms Cheung Siu
Ping
All cell function tests are performed after consultation with one of the senior
immunologists (Dr Brian Jones, 28554147; Dr Eric Chan, 28554149; Dr Janette
Kwok, 28554867). When it is agree which evaluations are appropriate, contact
the Cell Function Lab (28554178) to arrange a date for the test. On the agreed
date, a technician will meet the medical officer and the patient before 9:30
a.m. so that blood can be mixed in special bottles and transported immediately
to the laboratory. Blood must be taken aseptically. For most tests a control
sample is required ?this should be from an age- and sex- matched volunteer in
good health. These arrangements are for patients in Queen Mary Hospital - for
other hospitals the specimen must usually be delivered to the laboratory within
one hour of collection. Often it is best to arrange for the patient to come to
the laboratory for bleeding.
|
TESTS |
TAT
(DAYS) |
|
Lymphocyte subsets profile by flow cytometry |
1 |
|
Mitogen stimulated lymphocyte proliferation |
3 |
|
Antigen stimulated lymphocyte proliferation |
7 |
|
Neutrophil function
- myeloperoxidase |
1 |
|
- nitroblue tetrazolium reduction (NBT) |
1 |
|
-
Candida killing and phagocytosis |
1 |
|
-
Staphylococcus killing and phagocytosis |
7 |
|
- chemotaxis |
1 |
|
-
leucocyte adhesion glycoprotein (CD11b) |
1 |
|
- chemiluminescence |
1 |
|
Anti-neutrophil antibody |
1 (cells) 28 (serum) |
|
Natural killer cell function |
1 |
|
B-cell function and regulation |
8 |
|
Monocyte function |
2 |
|
Cytokine profile |
2 |
Each report will give
the patient's results, control results, the relevant normal ranges and an
immunologist's interpretation of the data.
The volume of
blood required will depend on the patient's white cell count and differential
and will be discussed during consultation.
|
TEST |
SPECIMEN |
|
Lymphocyte subsets |
EDTA (3 ml) |
|
Mitogen stimulated proliferation |
Sodium heparin |
|
Antigen stimulated proliferation |
Sodium heparin |
|
Neutrophil function
-
myeloperoxidase |
EDTA (3 ml) |
|
- NBT |
EDTA (3 ml) |
|
-
Candida killing and phagocytosis |
Sodium heparin |
|
-
Staph killing and phagocytosis |
Sodium heparin |
|
-
chemotaxis |
Sodium heparin |
|
-
CD11b |
EDTA (3 ml) |
|
-
chemiluminescence |
EDTA and sodium heparin |
|
Anti-neutrophil antibody |
EDTA and clotted (3 ml) (Clotted only if <106ml neutrophils) |
|
NK-cell function |
EDTA and sodium heparin |
|
B-cell function and regulation |
Sodium heparin |
|
Monocyte function |
Sodium heparin |
|
Cytokine profile |
Sodium heparin |